Efficacy and immunological actions of FAHF-2 in a murine model of multiple food allergies

In diesem Mausmodell konnte mit einer  seit 1800 Jahren verwendeten, minimal modifizierten Rezeptur  (FAHF-2  entspricht mod. Wu mei wan) aus dem Klassiker der chinesischen Medizin (Shan han lun) eine anaphylaktischen  Reaktion  bei Mäusen mit Nahrungsmittelallergie gegen Erdnuss, Kabeljau und  Ei -Allergie vormieden werden und auch die IgE Werte reduziert werden.

Background: Food Allergy Herbal Formula-2 (FAHF-2) prevents anaphylaxis in a murine model of peanut allergy. Multiple food allergies (MFA) are common and associated with a higher risk of anaphylaxis. No well-characterized murine model of sensitization to multiple food allergens exists, and no satisfactory therapy for MFA is currently available.

Objective: To determine the effect of FAHF-2 in a murine model of MFA.

Methods: C3H/HeJ mice were orally sensitized to peanut, codfish, and egg concurrently. Oral FAHF-2 treatment commenced 1 day after completing sensitization and continued daily for 7 weeks. Mice were subsequently orally challenged with each allergen.

Results: Antibodies in sera from mice simultaneously sensitized with peanut, codfish, and egg recognized major allergens of all 3 foods, demonstrating sensitization to multiple unrelated food allergens (MFA mice). Sham-treated MFA mice exhibited anaphylactic symptoms accompanied by elevation of plasma histamine and hypothermia. In contrast, FAHF-2-treated MFA mice showed no anaphylactic symptoms, normal body temperature, and histamine levels after challenge with each allergen. Protection was accompanied by reduction in allergen-specific immunoglobulin E levels. Allergen-stimulated Th2 cytokine interleukin-4 and interleukin-13 production levels decreased, whereas the Th1 cytokine interferon-γ levels were elevated in cultured splenocytes and mesenteric lymph node cells in FAHF-2-treated mice.

Conclusion: We established the first murine model of MFA. FAHF-2 prevents peanut, egg, and fish-induced anaphylactic reactions in this model, suggesting that FAHF-2 may have potential for treating human MFA.